This study aimed to develop a banNV to enhance immunogenicity against colorectal cancer. For this, dual adjuvants (TLR7/8 agonist: R848 and TLR9 agonist: CpG) and MC38 colorectal cancer cell–specific neoantigen (Adpgk) were programmably assembled and strategically encapsulated into multistructured nanoparticles, enabling efficient codelivery of adjuvants and neoantigens. To validate the immune activation induced by banNVs, in vitro APC stimulation and antigen presentation experiments and additional in vivo tumor prophylaxis and immunotherapy using MC38 tumor mouse models were designed. The number of mice per experimental group is indicated in the respective figure legends.

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