Data were statistically analyzed using GraphPad Prism 6 (GraphPad Software Inc., La Jolla, CA). Western blot and PCR densitometry data are presented as scatter dot plots with means ± SD superimposed. Differences between the two groups were evaluated using two-tailed unpaired Student’s t test. When the difference among three or more groups was evaluated, one-way analysis of variance (ANOVA) or, when appropriate, two-way ANOVA followed by Tukey’s multiple comparisons test or Dunnett’s multiple comparisons test was used. Serial echocardiography data were examined using two-way repeated measures ANOVA followed by Bonferroni’s test to assess interaction of time and drug intervention. For a dataset with unequal variances, two-tailed unpaired t test with Welch’s correction was also used and is indicated in figure legends.

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