Confocal microscopy was performed as described (11). Ventricular myocardium from GFPdgn tg mice was fixed with 3.8% paraformaldehyde and processed for obtaining cryosections. The myocardial sections (6 μm) were stained with Alexa Fluor 568–conjugated phalloidin (Invitrogen) to reveal F-actin and identify cardiomyocytes. GFPdgn direct fluorescence (green) and the stained F-actin (red) were visualized and imaged using a Leica TCS SP8 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).

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