To measure cytokine secretion, magnetic-activated cell sorting–purified CD4+ T cells (Miltenyi) from healthy donors were stimulated with anti-human plate-bound CD3 (1.0 μg/ml; clone OKT3, eBioscience) and anti-human soluble CD28 (0.5 μg/ml; clone CD28.2, eBioscience). GT13072 or GT14829 was added at the beginning of the culture. After 48 hours, the culture supernatants were taken for cytokine analysis. Cytokines were measured using the Meso Scale Discovery (MSD) platform according to the manufacturer’s instructions.

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