Total RNA was isolated using Trizol (Invitrogen) according to the manufacturer's instruction. Extracted RNAs were used for RT-PCR with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). qPCR was performed using QuantStudio 3 Real-Time PCR System. The primer sequences used for qRT-PCR were as follows: CDH6, 5′-GCCAGTGGCTCAAACTTTAC-3′ (forward) and 5′-GGTGCTCATCTCGTGTCTATTA-3′ (reverse); KCNK5, 5′-CATCATCTTCTACCTGGCCATC-3′ (forward) and 5′-GATGCAGCTTCTGTGTGTAGTA-3′ (reverse); PROM1, 5′-CATCTCTCAATGACCCTCTGTG-3′ (forward) and 5′-CCTCAGTTCAGGGTTGCTATT-3′ (reverse); AGPAT4, 5′-CAGCTTCATCCTCGTCTTCTT-3′ (forward) and 5′-TTCTGCTTGCTGTCAGAGTT-3′ (reverse); LAMA4, 5′-GACATCACAAGGAGAGGGAAAT-3′ (forward) and 5′-TCCATTGACCATCAGGAGAATAAG-3′ (reverse); NCAPH2, 5′-CCAAGCAGCTCTCTTCGGTG-3′ (forward) and 5′-CATCCAGCGACAGGAACTCA-3′ (reverse); DEPDC1B, 5′-GGAAATTCTGAAAGTCCCTTTGG-3′ (forward) and 5′-CCATATCAGCTCCTGGGTATTT-3′ (reverse); HSD17B2, 5′-CACCAGTGACAAGTGGGAAA-3′ (forward) and 5′-CCGCTGTGCTAAGATGTAGTC-3′ (reverse); MAP1B, 5′-CCCGTCAGGATGTCGATTTAT-3′ (forward) and 5′-CCCGTCAGGATGTCGATTTAT-3′ (reverse); PTPRC, 5′-AACTCTTGGCATTTGGCTTTG-3′ (forward) and 5′-CTGGGCATCTTTGCTGTAGT-3′ (reverse); UGT8, 5′-CTACAGTCCAAGAGCGGAATA-3′ (forward) and 5′-CCATCAGGTCACAGTTCTTAGT-3′ (reverse); and B2M, 5′-GGCATTCCTGAAGCTGACA-3′ (forward) and 5′-CTTCAATGTCGGATGGATGAAAC-3′ (reverse). B2M was used as an internal control.

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