Nuclear fractions were prepared following the IP protocol. The nuclear protein precipitate was resuspended in HEMG-0 buffer [25 mM Hepes (pH7.9), 0.1 M EDTA, 12.5 mM MgCl2, and 100 mM KCl]. One milligram of nuclear protein was carefully overlaid onto a 5-ml 20 to 50% sucrose gradient (in HEMG-0 buffer) or 10 to 30% glycerol gradient prepared in a 5-ml 13 mm × 51 mm polyallomer centrifuge tube (Beckman Coulter, 326819). Tubes were centrifuged in an SW-55 Ti swing-bucket rotor at 4°C for 16 hours at 100,000g. Fractions (0.4 ml) were collected for immunoblotting analyses.

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