OCCC cell line RMG1 cells were cultured in 1:1 Dulbecco’s modified Eagle’s medium (DMEM)/F12 supplemented with 10% fetal bovine serum (FBS). OCCC cell line TOV21G and OVCAR429 cells were cultured in RPMI 1640 with 10% FBS and 1% penicillin/streptomycin at 37°C, supplied with 5% CO2. Viral packing cells 293FT and Phoenix were cultured in DMEM with 10% FBS and 1% penicillin/streptomycin at 37°C, supplied with 5% CO2. TOV21G OCCC cells harbor a frameshift 1645insC mutation that leads to a complete loss of ARID1A protein expression (5). All the cell lines were authenticated at The Wistar Institute Genomics Facility using short tandem repeat DNA profiling. Mycoplasma testing was performed using LookOut Mycoplasma PCR detection (Sigma-Aldrich) every month. Transfection was performed using Lipofectamine 2000 (Life Technologies) following the manufacturer’s specifications.

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