Monoclonal rat anti-mouse CCR7 antibody IgG2A (clone no. 4B12, R&D Systems) was injected intravenously into wild-type mice (dose, 0.5 μg/g) 24 hours before UUO. Rat IgG2A isotype (R&D Systems) was injected as control. Equivalent doses of CCR7 antibody or rat IgG2A isotype were administered on days 0, 1, 3, and 5, and mice were euthanized on day 7 following UUO.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.