Monoclonal rat anti-mouse CCR7 antibody IgG2A (clone no. 4B12, R&D Systems) was injected intravenously into wild-type mice (dose, 0.5 μg/g) 24 hours before UUO. Rat IgG2A isotype (R&D Systems) was injected as control. Equivalent doses of CCR7 antibody or rat IgG2A isotype were administered on days 0, 1, 3, and 5, and mice were euthanized on day 7 following UUO.

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