Kidneys were fixed in 4% PFA for 24 hours and embedded in paraffin. Periodic acid–Schiff (PAS) staining was performed to evaluate pathological injury. Masson and Sirius red staining were performed to estimate renal fibrosis. Two blinded renal pathologists quantified staining in eight randomly selected fields. Data were analyzed using Image-Pro Plus software (Media Cybernetics, Rockville, MD, USA).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.