hOCs were detected using the Leukocyte TRAP Kit 387-A (Sigma-Aldrich) according to the manufacturer’s instructions. Briefly, cultured cells were fixed with formaldehyde for 5 min at room temperature, washed, and air-dried. After staining, TRAP-positive multinucleated cells were observed under a phase-contrast microscope at 20× magnification and digitally photographed.

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