NSG mice were subcutaneously injected with 1 × 106 Nalm6-eGFP-fLuc cells. When tumor volumes reached ~350 mm3, mice were injected with 2 × 106 CD19-tPSMA(N9del) CAR T cells. PET/CT imaging was performed as described above. Mice were euthanized on indicated dates, and the tumor, peripheral blood, and bone marrow were taken for further analyses.

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