NOD/SCID mice were subcutaneously injected with the indicated number of CD19-tPSMA(N9del) CAR T cells in 50 μl (50% Matrigel, Corning) into upper flank regions bilaterally. Mice were injected with 14.8 MBq of [18F]DCFPyL 30 min after injection of the cells. A whole-body, two-bed position PET/CT scan was performed using the SuperArgus scanner (Sedecal) with PET acquisition time of 5 min per bed position. The PET/CT images were processed using PMOD (v3.7; PMOD Technologies Ltd., Zurich, Switzerland). The PET images were first registered to the corresponding CT images of each mouse. The signal in the images due to renal uptake and bladder concentration of [18F]DCFPyL was masked for display using a thresholding method, where voxels inside the kidneys and the bladder with intensities higher than 2 to 5% of the maximum intensity were set to zero. After this, maximum intensity projections of the processed images were then generated as shown in Figs. 3, 4, and 6.

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