After euthanasia in a CO2 chamber, the peripheral blood was taken by cardiac puncture, and the bone marrow was taken from the femurs. Red blood cells (RBCs) were removed by RBC lysis buffer (BioLegend, San Diego, CA, USA). Cells were stained with α-PSMA antibody (catalog no. Ab77228, Abcam), α-hCD3 antibody (catalog no. 300330, BioLegend), α-CAR antibody (JRP0295, Juno Therapeutics), and 7-AAD (catalog no. 00-6993-50, eBioscience). Cells were analyzed with the BD LSR II Flow Cytometer using FACSDiva software (BD Biosciences). Obtained data were analyzed with FlowJo software (Tree Star).

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