As opposed to natural archaeal tethered lipids, which typically contain two transmembrane tethers to form a C-40 macrocyclic lipid (5), we synthesized and studied bipolar hemi-macrocyclic lipids (5) containing a single transmembrane tether between lipid tails; these are synthetically more accessible on the gram scale than fully macrocyclic archaeal lipids (25). Specifically, we generated three lipids that all contained a phytanyl acyl chain, an ether linkage to a glycerol, and a phosphocholine head group (fig. S1A). We chose phosphocholine as a head group to increase the probability of forming stable liposomes (26). We chose ether linkages to match the ether linkages that are found in archaea, which increase the chemical (i.e., hydrolytic) stability of lipids (6). We incorporated an untethered phytanyl acyl chain (as opposed to a saturated hydrocarbon chain without methyl groups) into all lipids to reduce the probability of a phase transition in the temperature range of interest (6) and to avoid phase transition–induced leakage (2729). This design made it possible to generate lipids T32, T36, and U16 in ~10 synthetic steps, as previously described (10).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.