The average contact pressure was calculated from the applied normal force and size of the load-bearing (or contact) area, derived from the imaging experiments. To derive the load-bearing area size, we thresholded the histogram of the deconvolved images (see example of the deconvolved image and intensity histogram in fig. S9, A and B) using an Otsu threshold (34).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.