As the image acquisition time is 100 ms and the sliding speeds range from 1 to 200 μm/s, the images of the bead during sliding can be slightly distorted. Distortion happened because the confocal microscopy setup scanned the image line by line and there was a certain delay time between each of the lines. Thus, the load-bearing area appears elongated along the sliding axis. As averaging of the thickness of the liquid happens within the load-bearing area, the average thickness measured during sliding was averaged over 100 ms (the image acquisition time).

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