A rheometer (Anton Paar DSR 301) was mounted on top of an inverted confocal microscope (Zeiss Axiovert 200M). We glued a sphere to the rheometer plate and made contact with the fluorescent liquid. The rheometer measures the normal force on the contact. The fluorescent liquid was excited by an argon ion continuous wave laser at 514 nm. The objective lens is a 63× 1.3 numerical aperture LD A-Plan (Zeiss). The filter sets used for filtering of excitation and emission light are dichroic mirror HFT 405/514 and emission filter BP 560|615. Imaging was performed using the Zeiss LSM 5 LIVE microscope control system.

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