The refractive index of the fluorescent liquid can potentially change when the liquid is confined. According to the model in (30), the expected change in refractive index with confinement is minimal (<1%) for film thicknesses larger than 10 nm. The potential change in refractive index increases to 10% for thinner films. This change can then result in an uncertainty in the gap measurements of up to 10%. Our conclusions are not affected by this uncertainty.

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