For C. elegans survival assays, animal survival was plotted as a nonlinear regression curve using the PRISM computer program (version 6, GraphPad Software Inc., La Jolla, CA). Survival curves were considered different than the appropriate control when P values were <0.05. Prism uses the product limit or Kaplan-Meier method to calculate survival fractions and the log-rank test (equivalent to the Mantel-Heanszel test) to compare survival curves. A two-sample t test for independent samples was used to analyze qRT-PCR results; P values of <0.05 are considered significant. All the experiments were repeated at least three times, unless otherwise indicated.

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