For C. elegans survival assays, animal survival was plotted as a nonlinear regression curve using the PRISM computer program (version 6, GraphPad Software Inc., La Jolla, CA). Survival curves were considered different than the appropriate control when P values were <0.05. Prism uses the product limit or Kaplan-Meier method to calculate survival fractions and the log-rank test (equivalent to the Mantel-Heanszel test) to compare survival curves. A two-sample t test for independent samples was used to analyze qRT-PCR results; P values of <0.05 are considered significant. All the experiments were repeated at least three times, unless otherwise indicated.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.