Assays were performed in 6-cm NGM plates. Five-microliter bacterial cultures (E. coli OP50 or P. aeruginosa PA14) were spotted at equal distances from the center of the plate and incubated at 37°C for 12 to 16 hours. Synchronized young adult animals [wild-type and npr-8(ok1439)] were placed at the center of the plate. At later times (4 and 24 hours), animals on each bacterial spot were scored and calculated to give a CI [CI = (number of animals on P. aeruginosa − number of animals on E. coli)/total number of animals] (36). A positive CI indicates preference for P. aeruginosa; a negative CI indicates preference for E. coli; and an index of zero indicates no preference between the two bacteria.

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