To quantify the CFUs of intestinal bacterial loads, synchronized L1 larvae [wild-type and npr-8(ok1439)] were grown on E. coli OP50 at 20°C until they reached the young adult stage. The animals were then transferred to plates seeded with P. aeruginosa/GFP and cultured at 25°C for 24 hours. To eliminate P. aeruginosa/GFP bound to the body, the animals were transferred to NGM plates seeded with E. coli and incubated for 15 min, followed by one more transfer to new NGM plates seeded with E. coli with 30-min incubation. Ten animals per condition were placed into 50-μl phosphate-buffered saline (PBS) with 0.1% Triton and then grounded. Serial dilutions of the lysates (10−1, 10−2, 10−3, 10−4) were plated onto LB/kanamycin plates to select for P. aeruginosa/GFP cells and incubated at 37°C for 24 hours. The CFUs on the plates were then counted.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.