Profiling of bacterial accumulation in the intestine
This protocol is extracted from research article:
Neuronal GPCR NPR-8 regulates C. elegans defense against pathogen infection
Sci Adv, Nov 20, 2019; DOI: 10.1126/sciadv.aaw4717

To determine the profiles of bacterial accumulation in the intestine, synchronized L1 larvae [wild-type and npr-8(ok1439)] were grown on E. coli OP50 at 20°C until they reached the young adult stage. The animals were then transferred to plates seeded with P. aeruginosa/GFP and cultured at 25°C for 24 hours. To eliminate P. aeruginosa/GFP bound to the body before microscopy, the animals were transferred to NGM plates seeded with E. coli and incubated for 15 min, followed by two more transfers to new NGM plates seeded with E. coli with 30-min incubation after each transfer. The animals were then visualized using a Zeiss Axio Imager fluorescence stereomicroscope.

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