Wild-type worms and mutants were maintained as hermaphrodites at 20°C and fed E. coli OP50 on modified nematode growth medium (NGM) agar plates (0.35% instead of 0.25% peptone) (44). The bacterial lawn used for C. elegans killing assays was prepared by spreading a 25-μl drop of an overnight culture of bacteria on modified NGM agar plates (3.5-cm-diameter petri plates). Plates were incubated at 37°C for 16 hours and then cooled down at room temperature for at least 1 hour before seeding with synchronized worms. Survival assays were performed at 25°C, and live worms were transferred daily to fresh plates. Worms were scored at the times indicated and were considered dead when they failed to respond to touch.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.