Wild-type worms and mutants were maintained as hermaphrodites at 20°C and fed E. coli OP50 on modified nematode growth medium (NGM) agar plates (0.35% instead of 0.25% peptone) (44). The bacterial lawn used for C. elegans killing assays was prepared by spreading a 25-μl drop of an overnight culture of bacteria on modified NGM agar plates (3.5-cm-diameter petri plates). Plates were incubated at 37°C for 16 hours and then cooled down at room temperature for at least 1 hour before seeding with synchronized worms. Survival assays were performed at 25°C, and live worms were transferred daily to fresh plates. Worms were scored at the times indicated and were considered dead when they failed to respond to touch.

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