Guide RNA target sequences were designed using online software TargetFinder ( and cloned into Bsm BI–digested lentiCRISPRv2 (Addgene, 52961) (65). Following lentiviral production, MCF7 cells were infected and selected with puromycin. Single clones were picked and genotyped by genomic DNA PCR. PCR products were cloned into the pGEM-T Easy vector (Promega, A1360) and sequenced.

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