Lentiviral shRNAs targeting human CTCF, LATS1, LATS2, YAP, and PFKFB3 were obtained from the pLKO.1-based TRC (The RNAi consortium) library (Addgene, #10878). Cells were infected with lentivirus and followed by puromycin selection. For reverse transcription PCR (RT-PCR), cells were lysed in TRIzol reagent (Invitrogen, #15596026), followed by total RNA purification. Reverse transcription of RNA was conducted using Moloney murine leukemia virus reverse transcriptase with random primers. Gene expression was determined by real-time quantitative PCR (qPCR) with the SYBR Green PCR Kit (Applied Biosystems, #4309155). Data were normalized against β-actin.

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