All Western blots were performed using isolated mitochondrial lysates. Following mitochondrial isolations, the mitochondrial pellets were suspended in radioimmunoprecipitation buffer containing protease inhibitor cocktails (Roche). The samples were then sonicated, and the insoluble fractions were discarded following centrifugation. SDS sample buffer was added to the lysates, and samples were boiled for 5 min. The samples were then loaded onto 10 to 15% acrylamide gels and then transferred onto polyvinylidene fluoride transfer membranes (MilliporeSigma). The following primary antibodies were used: Slc25a4 (ANT1) (Signalway Antibody; 32484; 1:500), Slc25a5 (ANT2) (Cell Signaling Technology; 14671; 1:500), ANT4 polyclonal antibody (Signalway Antibody; 40596-1; 1:500), and Total OXPHOS rodent WB antibody cocktail (that contained antibodies to complexes 1 to 5, ComII, or ComV as labeled in Western blots) (Abcam; ab110413; 1:15,000).

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