ChIP on both mouse and human samples was performed as described previously (49, 50), with the exception that amplification for the human ChIP samples was done with the New England Biolabs Ultra Amplification Kit (cat# E7370, NEB) and no Escherichia coli carrier DNA. Specific washing conditions not mentioned previously include four times low-salt (140 mM NaCl) radioimmunoprecipitation assay (RIPA) buffer washes for both H3K4me1 and H3K27ac precipitations, replacing all high-salt (500 mM) washes [see (50) for detailed protocol]. Abs used were CEBPA (clone 14AA, sc-61, Santa Cruz Biotechnology), H3K4me1 (ab8895, Abcam), and H3K27ac (ab4729, Abcam). See primer table (data file S7) for primer sets used for assessing the quality of each individual precipitation.

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