Recipient mice were transplanted with shRNA-transduced Lp30 cells (1.5 × 106 for each Nt5e knockdown construct and 0.5 × 106 for scrambled control). Three weeks after BMT, 5 × 105 GFP-sorted cells from two or three individual mice per construct were used for mRNA extraction (RNeasy mini kit, QIAGEN), and cDNA library was generated using 600 ng of input RNA (TruSeq Library RNA Prep Kit v2, Illumina). Libraries were diluted and sequenced (NextSeq, Illumina).

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