Total RNA was extracted from GMPs using the RNeasy Micro Kit (QIAGEN). Thirty nanograms of RNA was processed for double-stranded cDNA synthesis with the Ovation RNA-Seq System V2 (NuGEN Technologies). Subsequently, 1 μg of the sheared cDNA [fragment size, 200 to 600 base pairs (bp)] was subjected to library preparation according to the Illumina TruSeq DNA Sample Preparation Kit (ref #15012999) protocol. The indexed libraries were pooled in equimolar ratios and subjected to 100-bp paired-end sequencing on the Illumina HiSeq 2000 system.

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