For expression analysis, cells were sorted as described above, RNA-extracted using the RNeasy Micro Kit (QIAGEN) or NucleoSpin RNA XS (MN), and reverse-transcribed to cDNA using the ProtoScript First Strand cDNA Synthesis Kit (M-MuLV) and oligo (dT) primers (NEB). RT-qPCR was performed using LightCycler 480 SYBR Green I Master (Roche Life Sciences). Data were normalized to Actg1 (mouse) or POL2AR and H6PD (human). Primers used are listed in data file S7.

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