Sixty freshly collected B. solaris specimens were divided randomly into three different treatments using filtered seawater from the worm’s collection site: (i) 20 worms in filtered seawater, (ii) 20 worms in filtered seawater with 0.2 μg of 3-(4-chlorophenyl)-1-methoxy-1-methylurea (monolinuron) ml−1 added, (iii) 20 worms in filtered seawater with 2 μg of monolinuron ml−1 added. Individual worms were kept separately in 12-well culture plates with each well containing 2.5 ml of medium. All treatments were incubated at 12°C and illuminated at 65 μmol quanta m−2 s−1 under a 12-hour light/12-hour dark cycle for 2 weeks. Filtered seawater was refreshed daily. Worms were counted every day, and plastid retention was evaluated every week with the aid of a ZEISS Stemi 508 stereo microscope and a ZEISS Axiovert 200 inverted microscope equipped with a ZEISS Axiocam 503 color camera. Survival rates and plastid retention rates were calculated and visualized using Microsoft Excel.

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