Total RNA extraction and preparation of a full-length polyA-selected complementary DNA and sequencing library from a single specimen of B. solaris were done using the reagents and following the protocol in the Smart-seq2 pipeline (33). The specimen was starved for a week to avoid contamination with freshly ingested prey cells. Library quantity and quality were measured using a 2100 Bioanalyzer (Agilent) before sequencing. Paired-end sequencing was performed on an Illumina HiSeq 2500. The library was barcoded on one-fourth of a flow cell and sequenced with 125-bp paired-end reads (high-output mode).

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