Isolated specimens were pipetted onto a coverslip coated with poly-l-lysine and fixed with 2.5% (v/v) glutaraldehyde in filtered seawater for 1 hour. Glutaraldehyde was drawn off using a pipette, and specimens were post-fixed in 1% (w/v) osmium tetroxide with filtered seawater at 4°C for 1 hour. Specimens were rinsed with seawater and stuck to the coverslip using a large drop of low–melting point agar and then dehydrated through a graded ethanol series (30, 50, 75, 85, 90, 95, and 100%) at 10 min each. Specimens were washed in 1:1 ethanol:acetone for 10 min and 100% acetone for 10 min. After infiltration with a 1:1 acetone-resin mixture for 10 min, specimens were embedded in EPON 812 Resin for 12 hours, after which the resin was polymerized at 65°C for 24 hours.

Specimens were cut from the resin using a fine razor and glued to a resin stub in the desired orientation for sectioning. Ultrathin (45 nm) sections were cut using a DiATOME Ultracut diamond knife mounted on a Leica Ultracut E Ultramicrotome. Sections were placed on Formvar-coated copper grids and triple-stained with lead citrate for 10 min, uranyl acetate for 5 min, followed by lead citrate for 10 min. Stained grids were viewed under a Hitachi H7600 TEM with an accelerating voltage of 80 kV and photographed with an AMT XR50 CCD camera.

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