Specimens of B. solaris were collected between 2016 and 2018 from macroalgae in the rocky intertidal at Clover Point, Victoria, BC (48°24′12″N, 123°21′03″W) and West Beach, Calvert Island, BC (51°39′07″N, 128°08′33″W). Specimens of P. paranygulgus were collected in 2015, 2016, and 2018 from intertidal mudflats at Mud Bay Park, Surrey, BC (49°05′09″N, 122°51′39″W) and Ladysmith Inlet, Nanaimo, BC (49°01′28″N, 123°50′59″W). Live worms were isolated using the MgCl2 decantation method and the oxygen depletion method (31), mounted in seawater, and photographed using a ZEISS Axioplan 2 microscope equipped with differential interference contrast (DIC) optics and a ZEISS Axiocam 503 color camera. Live specimens were imaged using an Olympus FV1000 Multiphoton confocal laser scanning microscope (Olympus, Tokyo, Japan) at the University of British Columbia (UBC) Bioimaging Facility. Optical sections were taken from specimens excited at 635 nm (emission range, 650 to 750 nm). Optical sections and z-stack projection micrographs were compiled with Fiji version 2.00 (Wayne Rasband, National Institutes of Health).

Specimens of B. solaris and P. paranygulgus were used for TEM, genomic DNA extraction, and amplicon sequencing. The limited availability of P. paranygulgus prevented us from using specimens of this species for RNA sequencing (RNA-seq), as well as photosynthesis and starvation experiments.

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