Typically, 7.5 mg of GDL and 4.5 mg of CaCO3 were added in 1 ml of PE-GA/Pt 2 (3 wt %) aqueous suspension, and then, 10 mg of starch in 1 ml of sodium alginate (15 mg/ml) was added into the above solution to generate a hydrogel. The alginate-Ca2+ hydrogel containing PE-GA/Pt and starch was exposed to NIR light (3.8 W cm−2) for 20 min. After that, 200 μl of a solution consisting of TMB (1 mg/ml), HRP (10 μg/ml), GOx (10 μg/ml), and 0.2 M sodium acetate buffer (pH 4.5) was added to cover the gel surface. After 10 min, 0.1 M hydrochloric acid was added to adjust the pH. Last, the gel was imaged by an optical camera.

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