In a typical synthesis of PE-GA/Pt, GA/Pt was first modified with NAS. Ten milligrams of GA/Pt was dissolved in 10 ml of Hepes buffer (50 mM, pH 8.5), followed by dropwise addition of 200 μl of NAS in dimethyl sulfoxide (20 mg/ml). The reaction proceeded with magnetic stirring at 4°C for 3 hours. The product was then separated and purified via dialysis (3500 Da). The purified GA/Pt-NAS was dissolved in 10 mM PBS (pH 7.4), and then, 710 mg of AAm (10 mmol) was added to the solution. To synthesize PE-GA/Pt with different phase transition temperatures, different amounts of AN (265 mg for PE-GA/Pt 1, 292 mg for PE-GA/Pt 2, and 318 mg for PE-GA/Pt 3) were injected into the reaction solutions. For the synthesis of PE-GA/Pt 4, 20 mg of GA/Pt-NAS in 10 mM PBS (pH 7.4) was added with 710 mg of AAm and 305 mg of AN. After that, 30 mg of ammonium persulfate and 120 μl of tetramethylethylenediamine were added, and the reactions were conducted for 3 hours at room temperature. The products were collected by centrifugation (8000 rpm, 30 min) and washed 10 times by ultrapure water at different temperatures (10°C for PE-GA/Pt 1, 25°C for PE-GA/Pt 2, 45°C for PE-GA/Pt 3, and 37°C for PE-GA/Pt 4). The Pt contents in PE-GA/Pt were determined by inductively coupled plasma mass spectrometry (ICP-MS). PE-ProK/Pt and PE-DNase I/Pt were fabricated following the same protocol for the synthesis of PE-GA/Pt 2.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.