Larval nociception behavior assay was performed as described (12). Briefly, third instar larvae were transferred to a petri dish (10 cm) containing a thin film of distilled water. The larvae were allowed at least 10 min to habituate to the plate. A thermal heat probe (46°C) was touched to abdominal segments A4 to A6, and the response time was recorded as the time taken for the characteristic barrel roll response to occur. At least 60 animals were tested for each genotype.

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