The samples were prepared at the Geological Survey of Denmark and Greenland using standard palynological methods involving digestion of 20 g of sediment in hydrochloric and hydrofluoric acid, mild oxidation, and filtering of the organic residues over 11-μm mesh filter (60). Two to three strew slides were mounted per sample, using glycerine gel. To assess the percentage of aberrant forms, all normal and aberrant specimens of LTT- and LCT-spores were counted separately within one strew slide from each sample (tables S2 to S5), using a Leica DM 2000 transmitted light microscope fitted with a differential interference contrast system. During counting, the strew slides were scanned systematically using a ×40 lens (506097, Leica) along transverse transects, until the entire slide was scanned or until ≥100 specimens were counted. Only specimens that exhibit clear signs of aberrancy were counted as aberrant. Because folded, broken, or obscured specimens were always counted as normal, the obtained aberrancy values may be an underestimation. In some samples, statistical significance could not be obtained, and the results were disregarded in the plots in Figs. 3 to 5 and figs. S2 and S4. LCT-spores were generally much less abundant than the LTT-spores. The quantitative data are presented in tables S2 to S7.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.