Flies were reared on a standard corn meal, yeast, and sucrose agar medium at 25°C under a 12-hour/12-hour light/dark cycle. Canton S (BDSC 64349), painless [EP(2)2451] (BDSC 27895), ppk-Gal4 (BDSC 32078), UAS-CD8-GFP (BDSC 5130), UAS-Dcr2, UAS-tetanus toxin (active, BDSC 28838 and inactive BDSC 28839), UAS-p35 (BDSC 5072), and UAS-Lamin-GFP (BDSC 7376) flies were obtained from the Bloomington Drosophila Stock Center (BDSC) library. w1118 (VDRC 60000), UAS-TrpA1-RNAi (VDRC 37249), UAS-RDL-RNAi (VDRC 41101), UAS-GRD-RNAi (VDRC 5329), UAS-D-GABA-B-R1-RNAi (VDRC 101440), UAS-D-GABA-B-R2-RNAi (VDRC 110268 and VDRC 1785), UAS-D-GABA-B-R3-RNAi (VDRC 50176), UAS-LCCH3-RNAi (VDRC 37408), UAS-Vglut-RNAi (BDSC 27538), UAS-ChAT-RNAi (BDSC 25856), UAS-nAChRα1-RNAi (VDRC 48159), and UAS-twist-RNAi (VDRC 37091 and VDRC 37092) flies were obtained from the Vienna Drosophila Resource Center (VDRC) RNAi library (66). UAS-TrpA1 and TrpA1ins flies were from P. Garrity, and Gad1-Gal4 flies were from H. Bellen.

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