Skin bacteria were added to the surface of agar plates with one of the following treatments applied: nontreatment control, pristine sliver nanoparticles (AgNPs), and AAD with or without AgNPs. Plates were placed at 37°C, and bacterial growth was visualized in 3 days. For the encapsulation study, AgNP-laden AAD (diameter, 10 mm; thickness, 1.5 mm) was placed in 1 ml of Dulbecco’s phosphate-buffered saline (DPBS) solution at 37°C. The buffer was collected and replaced at certain time points (0.5, 2, 8, 24, and 48 hours and 7 days). A UV/visual spectroscopy with an absorbance at 420 nm was used to quantify the amount of AgNP released from AAD, and the calibration curve was measured with AgNP standards of 1, 0.5, 0.25, 0.125, 0.0625, and 0.03125 mg/ml. At every time point, there was no AgNP found in the release medium within the detectable range.

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