Chemical reagents used in this study were purchased from Sigma-Aldrich unless stated otherwise. The alginate was PRONOVA UP MVG purchased from FMC Corporation. Both nonthermoresponsive and thermoresponsive TA and AAD hydrogels were tested as wound dressings in this work. The nonthermoresponsive adhesives, called TA, consisted of alginate-polyacrylamide hydrogels, chitosan, and coupling reagents (e.g., EDC and NHS) (17). The thermoresponsive adhesives, called AAD, consist of a bilayered structure: a thermoresponsive antimicrobial dissipative matrix and a tissue-adhesive surface. The matrix was a PNIPAm-alginate hybrid network with embedded Ag-NPs for antimicrobial function, which was formed using N,N′-methylenebisacrylamide (MBAA) to covalently cross-link NIPAm and calcium sulfate (CaSO4) to ionically cross-link alginate. Briefly, 1.06 M NIPAm/0.083 M alginate solution in phosphate-buffered saline (PBS) was syringe mixed with 0.28 mM MBAA, 0.02 M CaSO4, 0.0065 M initiator ammonium persulfate, and 0.0037 M accelerator tetramethylethylenediamine. For antimicrobial function, 4 mg of AgNPs (diameter, 30 to 50 nm; US Research Nanomaterials Inc.) was mixed into the hydrogel precursors before gelation. For the hydrogels containing acrylamide (AAm), the molar percentages of AAm within the entire monomers (AAm plus NIPAm) were varied (0, 1, and 5%), as indicated above. The hydrogels were gelled inside a closed glass mold at either 4° or 20°C overnight. The adhesive surface was achieved by activating the hydrogel surfaces with chitosan (medium molecular weight) and carbodiimide reagents (e.g., EDC and NHS) according to a previously reported protocol (17). The concentration of chitosan was 2% (w/v); EDC and NHS were both 12 mg/ml. The mixture of chitosan, EDC, and NHS was applied to the surface of hydrogel matrix before application. For the hydrogels used in the in vivo experiments, the alginate and chitosan were sterile-filtered, frozen, and lyophilized for 1 week. All the other chemical agents such as NIPAm, AAm, and EDC were sterilized by filtering right before usage. The synthesis was conducted in a tissue culture hood. After gelation, the hydrogels were soaked in saline for 30 min and then rinsed three times before usage. The materials were stored at 4°C before use.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.