For the human dataset, scRNAseq data were integrated from both dissociation methods described above. Briefly, the top 12 cryopreserved samples were selected on the basis of their sampling of alveolar cell types (ATI and ATII cells), as determined by the standard Seurat clustering pipeline. The identified samples were then down-sampled to no greater than 1000 cells per identity to facilitate computational efficiency. A CCA was then performed between the fresh and cryopreserved human samples.

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