Cells were accepted with greater than 500 and fewer than 10,000 unique mapped genes. Analysis was performed on cells with less than 5% mitochondrial mapping for the mouse, rat, and pig samples and less than 8% for the human samples. Expression values were normalized to 10,000 transcripts per cell. Scaled values were calculated using Seurat v2.0’s ScaleData function with default settings, with regression of nUMI and percent mitochondrial transcripts.

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