To assess differences among mutant frequencies, the Mann-Whitney U test was applied. Nonparametric confidence intervals for medians were calculated as described (50). A two-tailed Fisher’s exact test was used to compare the relative proportions of specific types of mutation within sequenced cycA sample sets. In a different method, a Monte Carlo modification of the Pearson χ2 test of spectra homogeneity (51) was used to compare mutation distributions. Calculations were done using the COLLAPSE program (52), and correlation analyses were performed using the STATISTICA package.

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