TtClpP (3 μM) in a buffer containing 100 mM tris (pH 8.5), 100 mM NaCl, and 5% glycerol without or with 5 mM added bortezomib was measured using a DynaPro NANOSTAR (Wyatt, Santa Barbara, USA) instrument operated at 25° or 45°C and analyzed with the associated DYNAMICS software. Each experiment was performed in triplicate (three times for each of two TtClpP samples, both measured at 25° and 45°C), and hydrodynamic radii were calculated as the average of these replicates. Theoretical hydrodynamic radii of compressed TtClpP [based on PDB: 3QWD (18)] and extended TtClpP (using the TtClpP:bortezomib structure), after modeling of missing residues in the N-terminal β-hairpins and the N and C termini using SWISS-MODEL (63), were calculated using Hydropro10 (64) using a solvent viscosity and a solvent density of 0.0010387 poise and 1.0114 g/cm3, respectively (5% glycerol, 25°C), or 0.00068656 poise and 1.0043 g/cm3, respectively (5% glycerol, 45°C).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.