Separation of samples by size-exclusion chromatography was carried out in PBS buffer solution (pH 7.4) using an Agilent 1260 HPLC chromatograph (Agilent Technologies, USA) and Superdex 200 10/300 GL (24 ml) analytical column (GE Healthcare). For detection, successively online connected detectors were used: diode array detector Infinity 1260 detector operating at 280 nm (Agilent Technologies, USA), multiangle light scattering (MALS with 18 angles) photometer with GaAs linearly polarized laser (λ0 = 661 nm; DAWN HELEOS II), and Optilab rEX interferometric refractometer operating at the same wavelength as the photometer (both instruments from Wyatt Technology Corp., USA). The nominal eluent (PBS buffer) flow rate was 0.5 ml/min.

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