DNA from the progeny of the cross between BIL6521 and BIL6180 was extracted from leaf material that were archived on FTA (Flinders Technology Associates) PlantSaver cards (GE Healthcare, Uppsala, Sweden) and purified according to the manufacturer’s specifications. Extracted DNA from the FTA cards were used for PCR amplification with GoTaq Green Mastermix to genotype the sample using 04g011460_Marker_Indel-F/R and ASFF_Chr3_Indel_002_F/R (table S3).

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