The kidney specimens were embedded in 100% optimal cutting temperature (O.C.T.). Tissue sections or cells were blocked and incubated with primary antibody against Ser2P Pol II CTD (Abcam, ab-5095), Ser5P Pol II CTD (Abcam, ab-5131), fluorescein-DBA (Vector Lab, FL-1031), fluorescein-LTL (Vector Lab, FL-1321), or p-HEXIM1. After primary antibody incubation, sections were incubated with goat anti-rabbit Alexa Fluor 488 secondary antibody (Thermo Fisher Scientific). Sections were imaged using a fluorescence microscope (FV1000, Olympus).

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