Cells and tissue samples were lysed in radioimmunoprecipitation assay (RIPA) buffer with protease inhibitor cocktail, phosphatase inhibitors cocktail, and dithiothreitol (DTT). After incubating at 4°C for 30 min, cell or kidney lysates were cleared and denatured in SDS protein loading buffer. Protein samples were separated by SDS–polyacrylamide gel electrophoresis gels, transferred to nitrocellulose membranes, and immunoblotted with primary antibodies. The antibody information was listed in Supplementary Materials and Methods.

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