MRI scanning was performed on a 7.0-T PharmaScan 70/16 ultra shielding (US) (Bruker, Switzerland) equipped with a 40-mm radio frequency (RF) coil. Mice were anesthetized with 1.5 to 2.5% isoflurane inhalation. Kidneys were placed to the center of the RF coil. Respiratory sensor was used to monitor mice breathing, body temperature was maintained around 37°C. MRI scanning was gated using the respiratory triggering system. TKV was evaluated from contiguous images by manually encircling kidney parenchyma and summing up the products of areas and slice thickness. Parameters of MRI studies included coronal T2-Turbo RARE sequences; repetition time/echo time, 2970/33 ms; image size, 256 × 256; field of view, 40 mm by 40 mm; slice thickness, 0.5 mm; and slice numbers, 12 to 25 (depending on the kidney size).

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